FIGURE 2.
Effect of ROS modulators on cellular ROS. A, H460 cells were treated with the indicated concentrations of ROS modulators as shown in Fig. 1 and analyzed for ROS levels by flow cytometry using H2DCF-DA as a fluorescent probe. Data shown are relative fluorescence intensities over control level determined at 2 h post-treatment. B, ESR detection of ROS. H460 cells (1 × 106 cells/ml) were incubated in culture medium containing the spin trapper DMPO (100 mm) with or without FeSO4 (50 μm) and NaFM (5 mm). ESR spectra were recorded 10 min after the addition of the test agents. The spectrometer settings were as follows: receiver gain at 2.5 × 104, time constants at 0.04 s, modulation amplitude at 1.0 G, scan time at 42 s, magnetic field at 3475 ± 100 G. Data are the mean ± S.D. (n = 3). *, p < 0.05 versus nontreated control.