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. 2010 Oct 5;285(50):38832–38840. doi: 10.1074/jbc.M110.124958

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FIGURE 4. — Effects of Cav-1 knockdown on ROS-modulated cell migration, invasion, and Akt activity. A and B, shCav-1 and shCon cells were treated with DMNQ (5 μm), H₂O₂ (100 μm), and FeSO₄ (50 μm) for 24 h and analyzed for cell migration and invasion. C, shCav-1 and shCon cells were analyzed for Akt phosphorylation by Western blotting. Blots were probed with phospho-Akt Ser-473 antibody and reprobed with β-actin antibody. D, H460 cells were treated with DMNQ (5 μm), H₂O₂ (100 μm), and FeSO₄ (50 μm) and analyzed for Akt phosphorylation at 6 h post-treatment. Data are the mean ± S.D. (n = 3). *, p < 0.05 versus control cells.