FIGURE 5.

Desi expression in the integuments of Drosophila larvae during development. A, stage-dependent changes in Desi expression in the larval integuments. Shown are early wandering (EW.L3), within 1 h after initiation of wandering, and late wandering (LW.L3), ∼8 h after initiation of wandering. L2, second instar larvae; FL3, foraging 3rd instar larvae; PP, pupariated larvae; P, pupae. Data are given as means for six separate measurements using 16 animals each. ***, significantly different from the value of second instar larvae (p < 0.001, Tukey's HSD). B, environment-dependent changes in Desi expression in the larval integuments. Wandering larvae were continuously placed in a dry (15% RH) Petri dish (filled bars) or wet condition (on water absorbed cotton in Petri dish) (open bars). Some wandering larvae were transferred to the wet condition at the early wandering stage and thereafter returned to the dry condition (gray bar). Desi expression levels in wet condition were not changed with or without nutrition. Desi expression in the integuments of the larvae was measured at indicated time. * and **, significantly different from the value of wandering larvae (*, p < 0.05; **, p < 0.01, Tukey's HSD). Other explanations are as described in A.