Fig. 4.

Free energy landscapes. (Upper) Molten globule state of HLA reconstructed from the 11 landscapes determined from the NMR data corresponding to progressive denaturation in urea. (Lower) Free energy landscape for all-Ala, the protein without the four disulfide bonds; for this system the fully native state is not accessible under known experimental conditions. The stability of the all-Ala molten globule relative to that of the WT molten globule was estimated from the midpoint of the urea denaturation curve (F in kcal/mol and rmsd in Å).