HPLC analysis of the supernatant from strain CT-43, thuE− mutant BMB0545, complementary strain BMB0546 (thuE+), and BMB0547 (vector only). A, trace of the supernatant from strain CT-43. The position of the arrowhead is the characteristic peak of thuringiensin. B, trace of the supernatant from thuE− mutant BMB0545. C, trace of the supernatant from BMB0547 (vector only). D, trace of the supernatant from complementary strain BMB0546 (thuE+). The characteristic peak of thuringiensin is observed when the lack of expression of the ThuE enzyme is compensated for.