TABLE 1.

Bacterial strains and plasmids used in this study

Strains and plasmids	Features	Source/Reference
DH10B	E. coli(Δmrr_hsdRMS_mcrBC) mcrA recA1	New England Biolabs
BMB171	A plasmid-cured derivative of B. thuringiensis YBT-1463, which lacks the endogenous plasmid and the thu cluster	Ref. 43
CT-43	B. thuringiensis subsp. chinensis strain, no flagellum	Ref. 20
HD-2	B. thuringiensis subsp. thuringiensis strain	Ref. 8
EMB1228	E. coli recombinant strain with BAC plasmid harboring cry1Ba gene	This work
EMB1234	E. coli recombinant strain with BAC plasmid harboring cry1Ba gene	This work
BMB0542	B. thuringiensis recombinant strain producing thuringiensin	This work
BMB0543	B. thuringiensis recombinant strain producing thuringiensin	This work
BMB0545	Mutant of strain CT-43 in which the thuE gene was knocked out	This work
BMB0546	Recombinant of BMB0545 in which the knocked out thuE gene was retrocomplemented	This work
BMB0547	Recombinant of BMB0545 in which there was a pHT304 plasmid	This work
pMD19-T	PCR product cloning vector	TaKaRa
pHT304	E. coli–B. thuringiensis shuttle BAC vector	Ref. 26
pHT304-ts	pHT304 derivative with a thermosensitive replicon of B. thuringiensis	Ref. 24
pEMB0572	Plasmid-interrupted thuE gene by kanamycin gene inserted in pHT304-ts	This work
pEMB0557	An E. coli–B. thuringiensis shuttle BAC vector	Ref. 20
pBMB0558	Plasmid harboring cry1Ba gene of strain CT-43	This work