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Purification of recombinant C-His6-tagged FdmV from SB4030 as monitored by SDS-PAGE. Lane M, BenchmarkTM protein ladder (Invitrogen); lane 1, total soluble proteins; lane 2, partially purified FdmV after nickel-nitrilotriacetic acid affinity chromatography; lane 3, purified FdmV after HiTrapTM HP anion-exchange chromatography.
