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. 2010 Oct 6;285(50):38853–38860. doi: 10.1074/jbc.M110.147744

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© 2010 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 5. — In vitro characterization of FdmV as an amide synthetase that catalyzes FDM C and FDM M-3 amidation to afford FDM B and FDM M-6, respectively. A, chemical synthesis of FDM B from FDM C. B, FdmV-catalyzed amidation of FDM C and FDM M-3 to FDM B and FDM M-6, respectively. C, kinetic analysis of FdmV-catalyzed amidation of FDM C with variable FDM C and saturating l-Gln and ATP. D, FdmV-catalyzed amidation of FDM C utilizing l-Gln, free NH₃, or l-Asn as an ammonia source: completed assay with boiled FdmV as a control (I) and identical assays with l-Gln (II), CH₃CO₂NH₄ (III), or l-Asp (IV) as an ammonia source. ○, FDM C; ♢, FDM B.