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. 2010 Oct 6;285(50):39211–39223. doi: 10.1074/jbc.M110.156026

FIGURE 4.

FIGURE 4.

HCT15 cells preselected with butyrate, in vivo (Balb/SCID mice), induce more vascularized and proliferative tumors, generating of a functional VEGF:KDR autocrine loop, and exhibited a higher capacity of homing in lungs. A, subcutaneous inoculation of HCT15 cells treated with butyric acid gave rise to significantly bigger tumors (p = 0.0097) than non-treated HCT15 cells, treatment of mice, inoculated with butyrate-preselected HCT15 cells, with IMC-1C11 blocks the augmented tumor growth; B, immunoreactivity for phospho-Histone3 showed that tumors developed from HCT15 cells treated with butyric acid are significantly more proliferative (p = 0.02) than tumors from non-treated HCT15 cells, treatment with IMC-1C11 also decreases proliferation; C, immunoreactivity for CD31 in subcutaneous tumors showed that tumors developed from HCT15 cells preselected with butyric acid are significantly more vascularized (p < 0.0001) than tumors from non-treated HCT15 cells, treatment with IMC-1C11 also decreases vascularization; D, H&E from lung sections (quantified in the plot) showed that mice injected with HCT15 cells preselected with butyric acid developed significantly more lung homing spots than control cells. These data were obtained from three independent experiments (6 mice/experiment/experimental condition), with consistent results. *, statistical significance.