FIGURE 2.

SOCE in WT and JP1 KO myotubes. SOCE was evaluated by a Mn²⁺ quench technique in myotubes subjected to store depletion as described under “Experimental Procedures.” The arrow indicates the time point when the perfusion system was switched to a Mn²⁺-containing solution. In the left panel are shown the SOCE signals of WT myotubes and the effect of 5 μm BTP2. The right panel shows Mn²⁺ entry in JP1 KO myotubes in the absence or presence of 5 μm BTP2. Mean traces ± S.E. of at least 20 myotubes are shown.