FIGURE 5.
LRF/miR-28/miR-505/ASF/SF2 axis is modulated during serial passages of MEF in culture. a, densitometric quantification of Western blot and qRT-PCR quantification, respectively, of LRF and p19ARF in MEF passages. b and c, densitometric quantification of Western blot (b) and qRT-PCR quantification (c) of ASF/SF2 in MEF passages. b, top, an example of the Western blot analysis for ASF/SF2. d and e, qRT-PCR quantification of miRNA (d) and Lpp and Atp11c transcripts during MEF passages (e).