TABLE 1.
IL-8 (ng/mg protein) following CGN, TNF-α, and CGN plus TNF-α combined in NCM460 cells
Values are expressed as ng/mg cell protein ± S.D. (shown in parentheses). IL-8 results from combined exposure to CGN and TNF are in boldface type. Differences between additive value and combined exposure value are in italic type. Base-line level of IL-8 in the spent medium of untreated control samples after 24 h is 0.623 (0.061) ng/mg protein.
| Exposure to TNFα (24 h) | Exposure to CGN (24 h) | CGN-induced increase in IL-8 from base line | TNF-α−induced increase in IL-8 from base line | Sum of increases from CGN and from TNF-α | CGN and TNF-α in combination-induced increase in IL-8 from base line | Difference in IL-8 between sum of increases from CGN and from TNF-α versus CGN and TNF-α in combination | p value for differencea |
|---|---|---|---|---|---|---|---|
| ng/ml | μg/ml | ng/mg protein | ng/mg protein | ng/mg protein | ng/mg protein | ng/mg protein | |
| 0.01 | 1 | 0.73 (0.04) | 0.320 (0.02) | 1.05 (0.07) | 1.92 (0.10) | 0.87 (0.05) | 0.0002 |
| 0.05 | 1 | 0.73 (0.04) | 1.75 (0.03) | 2.48 (0.07) | 3.43 (0.16) | 0.95 (0.22) | 0.0007 |
| 0.1 | 1 | 0.73 (0.04) | 3.36 (0.22) | 4.09 (0.30) | 5.40 (0.22) | 1.31 (0.05) | 0.0037 |
| 1 | 1 | 0.73 (0.04) | 10.94 (0.31) | 11.67 (0.38) | 13.91 (0.10) | 2.24 (0.34) | 0.0006 |
| 2 | 1 | 0.73 (0.04) | 14.30 (0.85) | 15.03 (0.92) | 18.33 (0.36) | 3.30 (0.40) | 0.0044 |
| 5 | 1 | 0.73 (0.04) | 16.94 (0.54) | 17.67 (0.61) | 21.23 (0.77) | 3.56 (0.11) | 0.0033 |
a Differences in the IL-8 increase between the sum of increases from CGN and from TNF-α versus CGN and TNF-α in combination are compared by unpaired t test, two-tailed.