TABLE 3.

Enzymatic studies of V. alginolyticus PepD wild-type and mutant proteins

Residual activity determination for the hydrolysis of l-carnosine at 37 °C and pH 7.4 by the V. alginolyticus wild-type and mutants.

PepD	Related activity
	%
WT	100 ± 0.5
H80A	NDa
H82X (X = G,V,F,Y,H,E)	ND
D119X (X = A,E,L,R,F,P,M,I,S,C,N)	ND
N120A	ND
E149D	53.1 ± 3.6
E149X (X = A,G,I,S,H,W)	ND
E150D	60.4 ± 1.3
E150X (X = A,R,H)	ND
D173X (X = A,E)	ND
H219A	119.1 ± 1.5
N260A	60.6 ± 0.3
R369A	ND
S374A,S385A	113 ± 0.8
H461A	ND

^a ND, not determined.