FIGURE 3.

Poor expression of A2BR at the cell surface. Immunofluorescence localization of HA-A2BR and HA-A2AR in transfected AD-293 cells is shown. Cells were co-immunostained with rat mAb to HA and mouse mAbs against organelle markers. The bound primary antibodies were detected by using either Alexa Fluor 488 (green)- or Alexa Fluor 594 (red)-conjugated goat anti-rat or mouse IgG antibody. Cells were analyzed by immunofluorescence with confocal microscopy. Merged images are shown in yellow. CaNX, calnexin; γ-1, gamma subunit of AP-1; Na⁺/K⁺ ATPase, sodium/potassium ATPase; white bar, 10 μm.