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Effect of addition of serial DIV-S6 cysteine mutations on IUS produced by K1237E. Experiments were performed as in Fig. 1. Shown is the normalized time course of recovery from inactivation as in Fig. 3. To allow better discrimination between the graphs, a log scale was chosen for the time axis. The time course of recovery in K1237E/I1575C was significantly faster than in all other double mutants. n = 3–7.
