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Mutations of ϵArg2′ (ϵArg217) have little effect on the gating equilibrium constant. The small change in the diliganded gating equilibrium constant (E2, normalized by the WT value) indicates that this position is nearly isoenergetic between the ground state conformations. A, example clusters elicited by 20 mm choline (Cho). B, R/E analysis. Each point represents the average of three patches with its S.D. (Table 2). The WT is boxed.
