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Deletion of OGT abrogates the expression of HSP72, HSP90, and GRP78, but not the closely related chaperones GRP75 and HSC70. OGT wild-type and null cells (38 h post-OGT deletion) were heat stressed at 45 °C for 60 min, and recovered at 37 °C for 2, 4, or 8 h. Cells were harvested 41, 43, and 47 h post-OGT deletion. The expression of OGT, O-GlcNAc, and the HSPs HSP72, HSC70, GRP78, and GRP75 were assessed by immunoblot (IB) (n = 5). Actin is shown as a loading control.
