FIGURE 1.

Comparison of dNTP concentrations of human primary macrophages and activated PBMCs with steady state K_m and pre-steady state K_d values of HIV-1 RT to dNTP substrates. The dNTP concentrations of macrophages and activated PBMC, which were determined by LC-MS/MS and summarized in Table 1, are plotted with black bars. The gray bars represent dNTP concentrations of human primary macrophages and activated CD4⁺ T cells previously determined by the enzyme-based dNTP assay (22). The red and blue zones represent the ranges of the previously determined HIV-1 K_m (22, 37–39) and K_d (34, 40, 41) values of HIV-1 RT to dNTP substrates, respectively.