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. 2010 Oct 25;285(50):38751–38755. doi: 10.1074/jbc.C110.185777

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© 2010 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 3. — Suppressive effect of 1,25D3 is VDR-dependent, and cytokine production is independent from transcriptional inhibition. A, flow cytometry plots of naive CD4+ T cells after culture in Th17-polarizing conditions for 5 days. Cells were activated with PMA/Ionomycin for 4 h and then stained for IL-17 and IFNγ. B, measurement of IL-17, IL-17F, and IL-22 in the supernatants of the cultures described in A. C, real-time RT-PCR analysis of the Rorc, Rora, and Il23r genes from T cells after culture under Th17-polarizing conditions. D, comparison of ELISA versus mRNA expression levels of Il17, Il17f, and Il22. Data represent the means ± S.E. of at least three independent experiments.