TABLE 2.

Serine peptidase activity of recombinant L. major

The substrates consisted of the protecting groups Bz, Z, and Boc and the fluorescent group AMC. NC means that no cleavage was observed, and S.E. is the standard error of the mean.

Substrate	Km (±S.E.)	kcat (±S.E.)	kcat/Km
	μm	s−1	μm−1s−1
Bz-Arg-AMC	4.40 ± 0.55	0.016 ± 0.004	3.6 × 10−3
Z-Phe-Arg-AMC	3.07 ± 0.20	0.011 ± 0.001	3.6 × 10−3
Z-Arg-Arg-AMC	0.93 ± 0.16	0.010 ± 0.002	1.1 × 10−2
Boc-Gly-Arg-Arg-AMC	1.25 ± 0.22	0.013 ± 0.003	1.0 × 10−2
Z-Gly-Gly-Arg-AMC	3.77 ± 0.67	0.020 ± 0.008	5.3 × 10−3
Z-Gly-Pro-AMC	NC	NC	NC