Fig. 4.

DND1 binds a set of functionally related targets that are cell cycle regulators. (A) TAP-DND1 migrates at the predicted size of about 50 kDa (right lane) in a western blot of transfected 3T3 cells. (B) Compared with GFP alone (top panel), GFP-DND1 (green, lower panel) localizes to perinuclear granules in 3T3 cells. Scale bars: 20 μm. (C) qRT-PCR results from RNA immunoprecipitation (RIP) experiments using TAP-DND1 in 3T3 cells compared with the TAP tag alone and normalized to Hprt1. Bars represents fold-enrichment (the s.e.m. of at least three replicates) for each gene compared with the control. ^*P<0.05. n=the number of independent RIP replicates.