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. Author manuscript; available in PMC: 2011 Dec 1.
Published in final edited form as: Pharm Res. 2010 Sep 8;27(12):2528–2543. doi: 10.1007/s11095-010-0256-x

Fig. 4.

Fig. 4

Internalization and nuclear accumulation of pDNA in bEnd3 cells. (A) Synergistic effect of NLS and TAT-conjugates on cellular trafficking of rhodamine-labeled pDNA compacted by pLPEI at N/P ratio 15. Confocal microscopy images are shown in normal (upper panes) and lateral modes (lower panes). Red color: pDNA, blue color: nuclei stained with DAPI. (B) Nuclear accumulation of rhodamine-labeled pDNA depending on effects of NLS or mutant NLS (mNLS)-conjugates (ExGen500-polyplexes). (C) Synergistic effect of two peptide-conjugates on the expression of GFP pDNA. Confocal images of NLS (left) and NLS+ApoE (1:1, right) conjugates were obtained 16 h post-transfection IExGen500-polyplexes).