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. Author manuscript; available in PMC: 2011 Nov 3.
Published in final edited form as: Cell Metab. 2010 Nov 3;2(15):545–552. doi: 10.1016/j.cmet.2010.09.013

Figure 3. Deleting UCP2 in MCH neurons augments glucose sensing.

Figure 3

(A) Double immunohistochemistry / in situ hybridization for MCH peptide (DAB, brown stain) and Ucp2 mRNA (35S-labelled cRNA probe, silver grains) in wildtype mice. (B) Effects of UCP2 deletion on glucose sensing by MCH neurons. Dose response curve of glucose-stimulated depolarization of GFP-positive neurons from WT (Mch-Cre/Z/EG) and Mch-Cre/Ucp2lox/lox/Z/EG mice (mean ± SEM values above each point represent the number of neurons assessed, two-way ANOVA test, pinteraction=0.95, pcolume=0.02, prow=0.001). (C) Blood glucose levels of ad lib fed and overnight fasted, 8-week old male mice (mean ± SEM, n=8-10 per genotype). Asterisk, P<0.05; Two asterisks, P<0.01, compared with wildtype littermates (unpaired t Tests). (D) Representative glucose tolerance curves of 8-week-old male mice (mean ± SEM, n=8-10 per genotype, 2 g/Kg glucose i.p.). Asterisk, P<0.05; Two asterisks, P<0.01, compared with wildtype at a given time point (unpaired t Tests). (E) Insulin levels during glucose tolerance test. (mean ± SEM, n=8-10 per genotype, 2 g/Kg glucose i.p.).