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. Author manuscript; available in PMC: 2011 Oct 1.
Published in final edited form as: J Immunother. 2010 Oct;33(8):780–788. doi: 10.1097/CJI.0b013e3181ee6675

Figure 2. Transduction of ATCs with CCR2b or CCR7 augments migration to chemokines.

Figure 2

ATCs were transduced with SFG.CCR2b (A) or SFG.CCR7 (B) retrovirus and analyzed for expression of the transgene by flow cytometry using CCR2 or CCR7 antibodies. C) CCR2b or CCR7 transduced ATCs showed enhanced migration to CCL2 or CCL21, respectively, in transwell migration assays compared to ATCs transduced with the mismatched receptor. D) CCR2b transduced ATCs show improved migration to SK-N-SH (CCL2+) tumor supernatant, but not to IMR-32 or JF which do not secrete CCL2. E) ATCs transduced with CCR2b show enhanced transwell migration compared to non-transduced (NT) ATCs when cultured with tumor supernatants taken from neuroblastoma cell lines derived from primary patient tissue.