Figure 1. Single-channel activity evoked by capsaicin.
A, capsaicin (0.25 μm) applied in the pipette elicits single-channel activity in cell-attached patches from HEK293 cells transfected with hTRPV1 (Popen= 0.446), but not in non-transfected cells (top). The capsaicin-induced activity is inhibited in the presence of capsazepine (10 μm) in the bath solution (bottom trace, Popen= 0.025). Single-channel activity was recorded at a membrane potential of +60 mV, openings are upward and c denotes the closed state. B, plot of Popenvs. voltage shows that the open probability is voltage dependent (capsaicin concentration 0.5 μm; n= 4).