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. 2003 Dec 1;100(25):14846–14851. doi: 10.1073/pnas.2335201100

Fig. 3.

Fig. 3.

(A) GalNAc and acetylated azido GalNAc analogs: Ac4GalNAz, Ac42AzGal, and Ac46AzGalNAz. (B) Evaluation of acetylated azido GalNAc analogs (50 μM) for metabolic incorporation into CHO cell surface glycoproteins. Cells were incubated with the compounds, stained with phosphine-FLAG followed by a FITC-α-FLAG antibody, and analyzed by flow cytometry. (C) Dose-dependent incorporation of GalNAz into CHO cell surface glycoproteins. Cells were analyzed for cell surface azides as in B. (D) Competitive metabolism of 50 μM Ac4GalNAz and various concentrations of Gal, GlcNAc, or GalNAc. Cells were analyzed for cell surface azides as in B. MFI, mean fluorescence intensity in arbitrary units. Data points represent the average of duplicate experiments. Bars indicate high and low values.