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Analysis of transcytosed and serum Tg. WB (lane 1) or immunoprecipitation (lane 2) of purified Tgs (P), of Tgs collected from the upper (UC) or lower (LC) chamber of FRTL-5 cells in transcytosis assays. Immunoprecipitation of Tg in the thyroid extract (lane 1) or serum sample (lane 2) from a GD patient. Similar results were obtained in three additional patients.
