Fig. 3.
(a–c) Effect of heparitinase, but not of chondroitinase ABC, on: (a) HSPGs expression (assessed by flow cytometry), (b) transcytosis of unlabeled Tgs, and (c) relation between HSPGs expression and transcytosis in FRTL-5 cells. (d) Effect of an antibody against the sequence 2489–2503 (anti-rTgP) on Tg transcytosis across FRTL-5 cells. Tgs were applied alone or with anti-rTgP or RIgG. (e–h) The sequence 2489–2503 is exposed to a greater extent in low-horm-rTg. Horm-rTg (e and g), and low-horm-rTg (f and h) were subjected to WB after preadsorption with RIgG (lanes 1 and bars 1), preadsorption with RIgG and precipitation with anti-rTgP (lanes 2 and bars 2), preadsorption with rabbit anti-T4 (lanes 3 and bars 3), or preadsorption with anti-T4 and precipitation with anti-rTgP (lanes 4 and bars 4). (g and h) Pixel density of the bands in e and f.