TABLE 2.
Plasmids used in this study
| Plasmid | Description | Source |
|---|---|---|
| pVL438 | CEN URA3 CDC13 | Evans and Lundblad (1999) |
| pVL648 | CEN LEU2 CDC13 | Evans and Lundblad (1999) |
| pVL1084 | CEN LEU2 CDC13 | Pennock et al. (2001) |
| pVL690 | CEN LEU2 cdc13–E252K (cdc13-2) | Pennock et al. (2001) |
| pVL1519 | CEN LEU2 cdc13–S225A | This work |
| pVL3512 | CEN LEU2 cdc13–P235A | This work |
| pVL3516 | CEN LEU2 cdc13–F237A | This work |
| pVL1521 | CEN LEU2 cdc13–S249A | This work |
| pVL2570 | CEN LEU2 cdc13–S249A | This work |
| pVL3518 | CEN LEU2 cdc13–Q250A | This work |
| pVL3513 | CEN LEU2 cdc13–N251A | This work |
| pVL1727 | CEN LEU2 cdc13–E252A | This work |
| pVL3515 | CEN LEU2 cdc13–F253A | This work |
| pVL3517 | CEN LEU2 cdc13–N254A | This work |
| pVL2571 | CEN LEU2 cdc13–S255A | This work |
| pVL4351 | CEN LEU2 cdc13–Q256A | This work |
| pVL3335 | CEN LEU2 cdc13–S306A | This work |
| pVL4140 | CEN LEU2 cdc13–4Q→ 4Aa | This work |
| pVL5005 | CEN LEU2 cdc13–11Q→ 11Ab | This work |
| pVL2691 | URA3 cdc13-2 (integrating vector) | This work |
| pVL2619 | URA3 cdc13–S255A (integrating vector) | This work |
| pVL2693 | URA3 cdc13–S249A, S255A (integrating vector) | This work |
| pVL2859 | URA3 tel2-1 (integrating vector) | This work |
| pVL3277 | 2μ LEU2 CDC13-(myc)18 | This work |
| pVL2640 | 2μ LEU2 cdc13–S249A, S255A-(myc)18 | This work |
| pAB301 | CEN TRP1 ADH–GBD–CDC13–RDaa211-331 | Bianchi et al. (2004) |
| pAB313 | CEN TRP1 ADH–GBD–CDC13–RDaa211-331–E252K | Bianchi et al. (2004) |
| pVL3068 | CEN TRP1 ADH–GBD–CDC13–RDaa211-331–S255A | This work |
| pVL3070 | CEN TRP1 ADH–GBD–CDC13–RDaa211-331–S249A | This work |
| pVL693 | 2μ LEU2 ADH–GAL4–AD–EST1 | This work |
| pVL859 | 2μ LEU2 ADH–GAL4–AD–STN1aa5-494 | Chandra et al. (2001) |
| pVL3125 | 2μ TRP1 ADH–GAL4–DBD–CDC13R635A | Gao et al. (2007) |
| pVL3346 | 2μ TRP1 ADH–GAL4–DBD–CDC13R635A E252K | This work |
| pVL3345 | 2μ TRP1 ADH–GAL4–DBD–CDC13R635A S255A | This work |
| pVL3484 | 2μ TRP1 ADH–GAL4–DBD–CDC13R635A S249A | This work |
| pVL3527 | 2μ TRP1 ADH–GAL4–DBD–CDC13R635A P235S | This work |
| pVL3524 | 2μ TRP1 ADH–GAL4–DBD–CDC13R635A F237V | This work |
| pVL3525 | 2μ TRP1 ADH–GAL4–DBD–CDC13R635A F253A | This work |
| pVL3526 | 2μ TRP1 ADH–GAL4–DBD–CDC13R635A N254A | This work |
a
4Q→4A: cdc13–Q226A, Q250A, Q256A, Q307A.
b
11Q→11A: cdc13–Q66A, Q226A, Q250A, Q256A, Q307A, Q391A, Q476A, Q612A, Q644A, Q653A, Q828A.