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Typical folding of ovary-specific piRNA precursor from chromosome 19 (a) and CD spectral and absorbance (melting curve) of pre-piRNA with secondary structure (b) or DNA duplex as a control (c) at 260 nm in a temperature range from 28 to 84°C. Different colors represent various piRNAs derived from the predicted precursor. Dots indicate the start sites of piRNAs.
