Time course of N. crassa asexual sporulation. 0 hr, hyphae of the mycelial mat at the initiation of the experiment; 4 hr, nascent aerial hyphae with hyphal branching (arrows); 8 hr, minor constriction chains present (arrows); 10 hr, minor constriction chains common (arrows); 12 hr, major constriction chains abundant (arrows); 14 hr, free conidia present (arrows). Bar, 10 μm.