Figure 4.—

Separation-of-function slk19 alleles show consistent interactions with BIM1 or CLA4 deletions. (A) Haploid strains lacking BIM1, containing a deletion allele of slk19 or spo12Δ for a FEAR⁻ control and also carrying pRK44 [YCp–SLK19∷URA3] were streaked to single colonies on YPAD and replica plated onto 5-FOA–containing plates. Strains: slk19Δ, MATa (dKH274-1.4b) and MATα (dKH274-1.9d), slk19–ΔF, MATa (dKH268-7.7d) and MATα (dKH268-2.8b), slk19–ΔA, MATa (dKH262-6.4b) and MATα (dKH262-6.10b), and spo12Δ, MATa (dKH280-5.3b) and MATα (dKH280-5.7a). (B) Haploid cla4Δ strains carrying pRK44 [YCp-SLK19∷URA3] were grown to stationary phase in YPAD medium, serially diluted 10-fold in water, spotted onto 5-FOA medium, and grown at 30°. Strains: SLK19 (TKH223) and slk19Δ (dKH272-3.3a). (C) Ura⁻ (therefore they do not contain the SLK19 expressing plasmid), cla4Δ strains also containing a slk19 partial deletion (or spo12Δ, FEAR⁻ control) were isolated from tetrad dissection. These strains were serially diluted 10-fold in water, spotted onto YPAD medium, and allowed to grow at 34° or 24°. Strains: SLK19 (TKH223), slk19–ΔA (dKH260-5.1a), slk19–ΔF (dKH266-6.2c), and spo12Δ (dKH278-5.3b).