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Negative regulation of cytokinesis by Pab1. (A) Serial dilution of cdc16-116 pab1-4 double mutant and the single mutants to test for potential additive effects of SIN inhibitors growing at 25°, 30°, 32°, 34°, and 36°. (B) pab1-4, cdc16-116, and pab1 cdc16-116 cells were DAPI- and calcofluor-stained after 8 hr incubation at 32° (first three panels from left). DAPI-calcofluor staining of cdc16-116 cell at 36° (restrictive temperature for this strain) is also shown (far right panel).
