Figure 4.—

Genetic interactions for pab1-4 with SIN mutants. (A) Growth of cdc15-140, spg1-B8, cdc7-24, cdc11-119, cdc14-118, sid2-250, cdc16-116, mob1-R4, and sid1-239 septation mutants at their permissive (25°) and restrictive (36°) temperature. Double mutants were used to assay for suppression of lethality of these septation mutants by pab1-4, as indicated. Wild type (wt) and pab1-4 strains were used as a control. (B) Growth of spg1-B8, cdc11-119, cdc7-24, cdc14-11, and sid2-250 septation mutants at their permissive (25°) and restrictive (36°) temperature, assessed by serial dilution drop tests on plates (10-fold dilutions starting with 10⁴ cells). Moderated overexpression of pab1 (nmt41x-driven expression in the absence of thiamine) was used to assay for suppression of lethality by an excess of Pab1 in these septation mutants, as indicated.