A positive genetic selection scheme for mutations in D. virilis Sxl as suppressors of XSE-duplication–induced male lethality. Each Minos (Mi) transgene carried a copy of D. melanogaster sc and sisA in tandem,. The w+ marker on the X-linked transgene X1 generated orange eyes, while that of the autosomal transgene A1 generated red eyes. F0 males were exposed to 2700 rad. Of the 39,000 F1 females, 50% were mated in groups of 10, 20% in groups of 5, and 30% in groups of 2. The attached-X chromosome C(1)w was generated for the purpose of this scheme.