Figure 8.

β-AR activation induces a persistent increase in GluA1 phosphorylation at S845. (A) Slices from the same animal were either untreated (UT) or exposed to 1 µM ISO for 10 min. ISO-treated slices where then collected for analysis either immediately after ISO application or after perfusion with agonist-free aCSF for 60 or 120 min. GluA1 phosphorylation at S845 was significantly elevated compared with untreated controls at all time points tested (*P < 0.05 compared with UT, n = 6). In contrast, ISO had no effect on total GluA1 levels. (B) β-AR activation induced a transient activation of ERK1/2. Although phospho-ERK1/2 levels were significantly increased immediately after ISO application (*P < 0.05 compared with UT), they returned to control levels following ISO washout. Western blots were run using the same samples used for the experiments shown in panel A.