Table 1. Crystallization conditions of the proteins.
Protein | Protein solution | Reservoir solution |
---|---|---|
BamE (SeMet derivative) | 12.8 mg ml−1 in 10 mM HEPES pH 7.5, 100 mM NaCl, 3 mM MgCl2, 1 mM β-ME | 0.1 M HEPES pH 7.5, 10% 2-propanol, 20% PEG 4000 |
BamD | 30 mg ml−1 in 50 mM Tris pH 8, 1 M urea | 0.1 M HEPES pH 7.5, 20% PEG 6000 |
BamC-NT | 6.5 mg ml−1 in 10 mM HEPES pH 7.5, 100 mM NaCl, 20 mM KCl, 3 mM MgCl2, 1 mM β-ME, 5% glycerol | 0.1 M citric acid pH 3.5, 2 M (NH4)2SO4 |
BamC-CT | 45 mg ml−1 in 20 mM HEPES pH 7.5, 50 mM NaCl, 1 mM β-ME | 0.1 M MES pH 6.5, 25% PEG 1000 |