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. Author manuscript; available in PMC: 2010 Dec 7.
Published in final edited form as: Langmuir. 2009 Jul 7;25(13):7496–7505. doi: 10.1021/la9009898

Figure 4.

Figure 4

Force signature of stretched nebulin and force peak analysis. Examples of the mountain-range types of force spectra of C-NA4 bracketed nebulin with similar detachment lengths. The major peaks were subjected to WLC analysis to determine the persistence length and contour length for each. (A) Detachment force at 1000 pN and p ≈ 0.04 nm. (B) Detachment force at 660 pN and p ≈ 0.12 nm. (C) Detachment force at 220 pN and p ≈ 0.36 nm. Note that all major peaks have the same persistence length (p) but increasing contour length (L) by 75–200 nm as nebulin is stretched. Major peaks are identified () for periodicity analysis. The spacing between nearest peaks is Lpp. This set of curves thus arises from stretching 5, 3, and 1 nebulin molecule(s)