Figure 4. IFN-γ is preferentially induced by DSS treatment in the colon in PGRP-deficient but not in WT mice (A) and IFN- γ is required for full manifestation of colitis in PGRP-deficient mice (B).
A. Expression of 16 IFN genes was measured by qRT-PCR in the colons of PGRP-deficient and WT mice after 2 days of oral treatment with 5% DSS. The results are the ratio of the amount of mRNA in DSS-treated to untreated mice; means of 3 assays on 5 mice/group ± SEM; significance of differences between PGRP-deficient and WT mice is indicated by an asterisk, *, P<0.01. B. Higher survival of Pglyrp3−/− mice treated with neutralizing anti-IFN-γ mAbs than IgG-injected control mice following treatment with 5% DSS in drinking water for 11 days; 6 mice/group; *, P = 0.01.