Figure 5. Bacterial killing effect of CFTR in vivo and upregulation of cytokines, CFTR and CAII in E coli-infected rat prostate.

(A) Comparison of E coli bacterial activities recovered from rat prostatitis models without or with CFTR_inh-172 (10 µM). Each point indicates the bacterial CFU per gram of prostate tissue weight (***P<0.001). (B) E.coli up-regulated the expression of cytokine genes, CFTR and CAII in rat prostate as determined by RT-PCR. Data were from three experiments. (C) Expression of CFTR (160 kD) and CAII (29 kD) protein was significantly up-regulated in E.coli -infected rat prostate as determined by western blot. Data were from three experiments. (*P<0.05, **P<0.01, ***P<0.001).