Figure 8. Effect of HCO₃ ⁻ and pH on expression of E. coli initiation factors IF1, IF2 and IF3.

(A) The mRNA expression of initiation factors was significantly inhibited by 80 mM HCO₃ ⁻. (B) Alkaline pH with a constant HCO₃ ⁻ of 25 mM did not suppress IF1, IF2 and IF3 gene expression. 16 s was used as control. Data were from three experiments. (*P<0.05).