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. 2010 Dec 7;5(12):e15255. doi: 10.1371/journal.pone.0015255

Table 1. Primers and RT-PCR conditions.

Primer name Sequence(5′→3′) Length(bp) Annealing temperature(°C)
GAPDH ForwardReverse GACCACAGTCCATGCCATCACTGC GCTGTTGAAGTCGCAGGAGACAAC 340 55
CFTR ForwardReverse AACTGAGACCTTACGCAG AGAAGCTCTGGTCCTCTG 481 55
CAII ForwardReverse ATGACCCTTCCCTACAGC GGTCACACATTCCAGCAG 503 56
IL-1β ForwardReverse CAACAAAAATGCCTCGTGC TGCTGATGTACCAGTTGGG 313 58
IL-6 ForwardReverse AAATCTGCTCTGGTCTTCTGG TTAGATACCCATCGACAGG 414 55
TNF-α ForwardReverse TACTGAACTTCGGGGTGATCG CCT TGTCCCTTGAAGAGAACC 292 58
E.coli 16S ForwardReverse CTCCTACGGGAGGCAGCAGGWATTACCGCGGCKGCTG 162 55
E.coli IF1 ForwardReverse ATGGCCAAAGAAGACAATATTG AGCGACTACGGAAGACAATG 216 55
E.coli IF2 ForwardReverse TTTCCGCTTCAATCACTTTAC CCTTGCTGAAACTGTCTACTG 165 55
E.coli IF3 ForwardReverse AAAGGCGGAAAACGAGTTC CCTTACTGTTTCTTCTTAGGAGCG 541 55