Table 1. Primers and RT-PCR conditions.
Primer name | Sequence(5′→3′) | Length(bp) | Annealing temperature(°C) | |
GAPDH | ForwardReverse | GACCACAGTCCATGCCATCACTGC GCTGTTGAAGTCGCAGGAGACAAC | 340 | 55 |
CFTR | ForwardReverse | AACTGAGACCTTACGCAG AGAAGCTCTGGTCCTCTG | 481 | 55 |
CAII | ForwardReverse | ATGACCCTTCCCTACAGC GGTCACACATTCCAGCAG | 503 | 56 |
IL-1β | ForwardReverse | CAACAAAAATGCCTCGTGC TGCTGATGTACCAGTTGGG | 313 | 58 |
IL-6 | ForwardReverse | AAATCTGCTCTGGTCTTCTGG TTAGATACCCATCGACAGG | 414 | 55 |
TNF-α | ForwardReverse | TACTGAACTTCGGGGTGATCG CCT TGTCCCTTGAAGAGAACC | 292 | 58 |
E.coli 16S | ForwardReverse | CTCCTACGGGAGGCAGCAGGWATTACCGCGGCKGCTG | 162 | 55 |
E.coli IF1 | ForwardReverse | ATGGCCAAAGAAGACAATATTG AGCGACTACGGAAGACAATG | 216 | 55 |
E.coli IF2 | ForwardReverse | TTTCCGCTTCAATCACTTTAC CCTTGCTGAAACTGTCTACTG | 165 | 55 |
E.coli IF3 | ForwardReverse | AAAGGCGGAAAACGAGTTC CCTTACTGTTTCTTCTTAGGAGCG | 541 | 55 |