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. 2010 Dec 7;5(12):e15207. doi: 10.1371/journal.pone.0015207

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Ruhe, Hayes.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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His₆-GalE chains were purified from tmRNA(DD) cells and analyzed by Western blot using anti-SsrA(DD) polyclonal antibodies. The His₆-GalE(recode) protein was expressed from a construct in which the last 170 codons had been synonymously recoded to change the mRNA sequence, but not the protein sequence (see Fig. S1). His₆-GalE(AK) contains two additional residues (Ala-Lys) at its C-terminus. His₆-GalE chains in the lanes labeled P_T7 and P _araBAD were synthesized from bacteriophage T7 and E. coli RNA polymerase expression systems, respectively.