Table 2. Plasmids used in this study.
Plasmids | Descriptiona | Reference |
pGalET́ | pET21b-derived plasmid expressing GalE, Ampr | This study |
pGalE(trp-At) | pET21b-derived plasmid containing the trp attenuator-terminator downstream of galE, Ampr | This study |
pHis6-GalE | pET21b-derived plasmid expressing His6-GalE, Ampr | This study |
pHis6-GalE(trp-At) | pET21b-derived plasmid containing the trp attenuator-terminator downstream of his6-galE, Ampr | This study |
pHis6-GalE(recode) | pET21b-derived plasmid expressing His6-GalE from recoded galE gene, Ampr | This study |
pHis6-GalE(AK) | pET21b-derived plasmid expressing His6-GalE with C-terminal Ala-Lys extension, Ampr | This study |
pBAD-His6-GalE | pBAD24-derived plasmid expressing His6-GalE, Ampr | This study & [64] |
pHis6-TrxA | pET21b-derived plasmid expressing His6-TrxA, Ampr | This study |
pHis6-GalE(1–180)-TrxA | pET21b-derived plasmid expressing His6-GalE(1–180)-TrxA, Ampr | This study |
pHis6-TrxA-GalE(180–338) | pET21b-derived plasmid expressing His6-TrxA-GalE(180–338), Ampr | This study |
pCH450 | pACYC184-derived plasmid containing E. coli araC and ParaBAD, Tetr | [9] |
pRNase II | pCH450-derived plasmid expressing RNase II under control of ParaBAD, Tetr | [9] |
pPNPase | pCH450-derived plasmid expressing PNPase under control of ParaBAD, Tetr | [9] |
pRNase R | pCH450-derived plasmid expressing RNase R under control of ParaBAD, Tetr | [9] |
pRARE | pACYC184-derived plasmid expressing E. coli tRNA4 Arg, tRNA5 Arg, tRNA2 Gly, tRNA2 Ile, tRNA3 Leu, and tRNA2 Pro, Cmr | Novagen |
pHis6-GalE(1–141)-SsrA(DD) | pET21b-derived plasmid expressing His6-GalE(1–141)-SsrA(DD), Ampr | This study |
pHis6-GalE(1–193)-SsrA(DD) | pET21b-derived plasmid expressing His6-GalE(1–193)-SsrA(DD), Ampr | This study |
pHis6-GalE(1–337)-SsrA(DD) | pET21b-derived plasmid expressing pHis6-GalE(1–337)-SsrA(DD), Ampr | This study |
pHis6-GalE(1–180)-TrxA(1–12)-SsrA(DD) | pET21b-derived plasmid expressing His6-GalE(1–180)-TrxA(1–12)-SsrA(DD), Ampr | This study |
pHis6-TrxA-GalE(180–193)-SsrA(DD) | pET21b-derived plasmid expressing His6-TrxA-GalE(180–193)-SsrA(DD), Ampr | This study |
pHis6-TrxA-GalE(180–337)-SsrA(DD) | pET21b-derived plasmid expressing His6-TrxA-GalE(180–337)-SsrA(DD), Ampr | This study |
pHis6-TrxA-SsrA(DD) | pET21b-derived plasmid expressing His6-TrxA-SsrA(DD), Ampr | This study |
Abbreviations used: ampicillin resistant, Ampr; chloramphenicol resistant, Cmr; tetracycline resistant, Tetr.