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. 2010 Dec 7;5(12):e15207. doi: 10.1371/journal.pone.0015207

Table 2. Plasmids used in this study.

Plasmids Descriptiona Reference
pGalET́ pET21b-derived plasmid expressing GalE, Ampr This study
pGalE(trp-At) pET21b-derived plasmid containing the trp attenuator-terminator downstream of galE, Ampr This study
pHis6-GalE pET21b-derived plasmid expressing His6-GalE, Ampr This study
pHis6-GalE(trp-At) pET21b-derived plasmid containing the trp attenuator-terminator downstream of his6-galE, Ampr This study
pHis6-GalE(recode) pET21b-derived plasmid expressing His6-GalE from recoded galE gene, Ampr This study
pHis6-GalE(AK) pET21b-derived plasmid expressing His6-GalE with C-terminal Ala-Lys extension, Ampr This study
pBAD-His6-GalE pBAD24-derived plasmid expressing His6-GalE, Ampr This study & [64]
pHis6-TrxA pET21b-derived plasmid expressing His6-TrxA, Ampr This study
pHis6-GalE(1–180)-TrxA pET21b-derived plasmid expressing His6-GalE(1–180)-TrxA, Ampr This study
pHis6-TrxA-GalE(180–338) pET21b-derived plasmid expressing His6-TrxA-GalE(180–338), Ampr This study
pCH450 pACYC184-derived plasmid containing E. coli araC and ParaBAD, Tetr [9]
pRNase II pCH450-derived plasmid expressing RNase II under control of ParaBAD, Tetr [9]
pPNPase pCH450-derived plasmid expressing PNPase under control of ParaBAD, Tetr [9]
pRNase R pCH450-derived plasmid expressing RNase R under control of ParaBAD, Tetr [9]
pRARE pACYC184-derived plasmid expressing E. coli tRNA4 Arg, tRNA5 Arg, tRNA2 Gly, tRNA2 Ile, tRNA3 Leu, and tRNA2 Pro, Cmr Novagen
pHis6-GalE(1–141)-SsrA(DD) pET21b-derived plasmid expressing His6-GalE(1–141)-SsrA(DD), Ampr This study
pHis6-GalE(1–193)-SsrA(DD) pET21b-derived plasmid expressing His6-GalE(1–193)-SsrA(DD), Ampr This study
pHis6-GalE(1–337)-SsrA(DD) pET21b-derived plasmid expressing pHis6-GalE(1–337)-SsrA(DD), Ampr This study
pHis6-GalE(1–180)-TrxA(1–12)-SsrA(DD) pET21b-derived plasmid expressing His6-GalE(1–180)-TrxA(1–12)-SsrA(DD), Ampr This study
pHis6-TrxA-GalE(180–193)-SsrA(DD) pET21b-derived plasmid expressing His6-TrxA-GalE(180–193)-SsrA(DD), Ampr This study
pHis6-TrxA-GalE(180–337)-SsrA(DD) pET21b-derived plasmid expressing His6-TrxA-GalE(180–337)-SsrA(DD), Ampr This study
pHis6-TrxA-SsrA(DD) pET21b-derived plasmid expressing His6-TrxA-SsrA(DD), Ampr This study
a

Abbreviations used: ampicillin resistant, Ampr; chloramphenicol resistant, Cmr; tetracycline resistant, Tetr.