Figure 5. RNA gel blot analysis of expression of VdGARP1 transcript under different conditions.

(A) Expression of VdGARP1 transcript under stress conditions by adding sorbitol or NaCl to three-day-old V592 liquid culture, or by transfer three-day-old V592 liquid culture to liquid medium lacking glucose or nitrate. (B) Expression of VdGARP1 mRNA stimulated by cotton, by adding cotton root extract to three-day-old V592 liquid culture. Co-culture times are as indicated. rRNAs stained with methylene blue trihydrate were used as a loading control. Hybridization was performed with ³²P-labeled VdGARP1-specific DNA probe as shown in Figure 3A.