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. 2010 Dec 7;4(12):e905. doi: 10.1371/journal.pntd.0000905

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Real et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Time-lapse recording of macrophages infected with L. amazonensis-WT for 48 hours and superinfected with metacyclic-enriched L. major-DsRed2 promastigotes. Image acquisition started 6 days after L. major-DsRed2 promastigote addition. Division of L. major-DsRed2 promastigote (arrowheads) inside L. amazonensis-WT PV (asterisk) was documented. The figure shows phase contrast (Ph2) in the first row, DsRed2 signal in the second, and Lysotracker merged with DsRed2 signal in the third. Time after promastigote addition is shown (d:h:min). Scale at 10 µm.