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. 2010 Nov 5;7:93. doi: 10.1186/1742-4690-7-93

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Copyright ©2010 Li and Lynch; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Structures of the amphotropic virus and retroviral vectors introduced into NSCs for pseudotyping and trans complementation analysis of CasBrE env genes. C17.2 NSCs were transduced with the pSFF-based retroviral vectors encoding CasBrE SU/TM (CasE), CasBrE SU (CasES), or the humanized renilla green fluorescent protein (hrGFP; striped) followed by infection with the amphotropic virus 4070A. The complementing viral structural elements are shown as filled regions for the 4070A virus and CasE and CasES vectors. The hrGFP vector served as a vector control that could be followed by fluorescence microscopy. Vector elements designated with a Δ represent deleted retroviral structural elements. Ψ indicates the presence of retroviral packaging sequences. SD, Splice donor. SA, Splice acceptor. LTR, Long terminal repeat.