Table 2.
MALDI-TOF/TOF (MS/MS) analysis of all nine overexpressd proteins in SM resistant M. tuberculosis clinical isolates
| Spot No. | Peak Mass (Da) | Protein Identified | Nominal mass | Mascot Score | pI | Sequence of peptide | ORF No. |
|---|---|---|---|---|---|---|---|
| 1 | 1062.611 | Chaperone protein dnaK | 66790 | 33 | 4.85 | RTTPSIVAFARN | Rv0350 |
| 1226.711 | Chaperone protein dnaK | 66790 | 34 | 4.85 | KDAGQIAGLNVLRI | Rv0350 | |
| 1567.931 | Chaperone protein dnaK | 66790 | 61 | 4.85 | KLLGSFELTGIPPAPRG | Rv0350 | |
| 1645.962 | Chaperone protein dnaK | 66790 | 40 | 4.85 | RIVNEPTAAALAYGLDKG | Rv0350 | |
| 2613.426 | Chaperone protein dnaK | 66790 | 60 | 4.85 | RSETFTTADDNQPSVQIQVYQGERE | Rv0350 | |
| 2 | 940.555 | 60 kDa Chaperonin 2 | 56692 | 58 | 4.85 | KDLLPLLEKV | Rv0440 |
| 1503.710 | 60 kDa Chaperonin 2 | 56692 | 45 | 4.85 | KGYISGYFVTDPERQ | Rv0440 | |
| 1658.900 | 60 kDa Chaperonin 2 | 56692 | 89 | 4.85 | KQIAFNSGLEPGVVAEKV | Rv0440 | |
| 1790.868 | 60 kDa Chaperonin 2 | 56692 | 20 | 4.85 | KDETTIVEGAGDTDAIAGRV | Rv0440 | |
| 2075.110 | 60 kDa Chaperonin 2 | 56692 | 65 | 4.85 | KTDDVAGDGTTTATVLAQALVRE | Rv0440 | |
| 2203.240 | 60 kDa Chaperonin 2 | 56692 | 114 | 4.85 | KKTDDVAGDGTTTATVLAQALVRE | Rv0440 | |
| 2317.275 | 60 kDa Chaperonin 2 | 56692 | 62 | 4.85 | KVVVTKDETTIVEGAGDTDAIAGRV | Rv0440 | |
| 3 | 1693.989 | Malate Dehydrogenase | 34358 | 52 | 4.65 | RLASGSLLGPDRPIELRL | Rv1240 |
| 1722.957 | Malate Dehydrogenase | 34358 | 38 | 4.65 | KVAVTGAAGQIGYSLLFRL | Rv1240 | |
| 1879.944 | Malate Dehydrogenase | 34358 | 56 | 4.65 | KGGNWTIVSGLEIDEFSRG | Rv1240 | |
| 2423.360 | Malate Dehydrogenase | 34358 | 52 | 4.65 | RVGVTGNPANTNALIAMTNAPDIPRE | Rv1240 | |
| 4 | 995.545 | Hypothetical Protein Rv3075c | 33194 | 30 | 4.73 | RLAFGIGDFRR | Rv3075c |
| 1016.503 | Hypothetical Protein Rv3075c | 33194 | 26 | 4.73 | KEFFAEFARD | Rv3075c | |
| 1322.619 | Hypothetical Protein Rv3075c | 33194 | 63 | 4.73 | RWFGDGNADWVRI | Rv3075c | |
| 1491.919 | Hypothetical Protein Rv3075c | 33194 | 32 | 4.73 | RLPNVPIVALVETARG | Rv3075c | |
| 1583.780 | Hypothetical Protein Rv3075c | 33194 | 64 | 4.73 | RDTGFGEDPATLAYARS | Rv3075c | |
| 1648.036 | Hypothetical Protein Rv3075c | 33194 | 45 | 4.73 | KRLPNVPIVALVETARG | Rv3075c | |
| 5 | 897.627 | Probable Oxidoreductase | 30516 | 15 | 4.70 | KTPAQVLLRW | Rv2971 |
| 1105.658 | Probable Oxidoreductase | 30516 | 27 | 4.70 | KLATPDQGFTRS | Rv2971 | |
| 1368.911 | Probable Oxidoreductase | 30516 | 72 | 4.70 | RWNLQLGNAVVVRS | Rv2971 | |
| 1382.701 | Probable Oxidoreductase | 30516 | 12 | 4.70 | RWNLQLGNAVVVRS | Rv2971 | |
| 6 | 1069.609 | Electron transfer flavoprotein subunit alpha | 31672 | 43 | 4.71 | KVAPQLTEAIKA | Rv3028c |
| 1341.748 | Electron transfer flavoprotein subunit alpha | 31672 | 56 | 4.71 | RIGSGLLVDVVDVRE | Rv3028c | |
| 1577.902 | Electron transfer flavoprotein subunit alpha | 31672 | 113 | 4.71 | MAEVLVLVEHAEGALKK | Rv3028c | |
| 1706.093 | Electron transfer flavoprotein subunit alpha | 31672 | 65 | 4.71 | MAEVLVLVEHAEGALKKV | Rv3028c | |
| 1973.063 | Electron transfer flavoprotein subunit alpha | 31672 | 69 | 4.71 | RAAVDSGYYPGQFQVGQTGKT | Rv3028c | |
| 2024.322 | Electron transfer flavoprotein subunit alpha | 31672 | 67 | 4.71 | KTVSPQLYIALGISGAIQHRA | Rv3028c | |
| 2692.697 | Electron transfer flavoprotein subunit alpha | 31672 | 14 | 4.71 | KNGLVLVIDGQLWTITEFQHVKPGKG | Rv3028c | |
| 7 | 1088.702 | Antigen 84 | 28260 | 22 | 4.80 | RLIEENSDLRQ | Rv2145c |
| 1171.748 | Antigen 84 | 28260 | 32 | 4.80 | RANAEQILGEARH | Rv2145c | |
| 1807.228 | Antigen 84 | 28260 | 38 | 4.80 | RLKTYLESQLEELGQRG | Rv2145c | |
| 1817.241 | Antigen 84 | 28260 | 33 | 4.80 | RVLSLAQDTADRLTNTAKA | Rv2145c | |
| 8 | 885.507 | 14 kDa antigen | 16217 | 21 | 5.00 | MATTLPVQRH | Rv2031c |
| 1162.563 | 14 kDa antigen | 16217 | 47 | 5.00 | RSEFAYGSFVRT | Rv2031c | |
| 1715.053 | 14 kDa antigen | 16217 | 28 | 5.00 | KGILTVSVAVSEGKPTEKH | Rv2031c | |
| 1869.098 | 14 kDa antigen | 16217 | 46 | 5.00 | RAELPGVDPDKDVDIMVRD | Rv2031c | |
| 9 | 929.516 | Hypothetical protein Rv0569 | 9517 | 22 | 5.70 | KVGDWLVIKG | Rv0569 |
| 1109.493 | Hypothetical protein Rv0569 | 9517 | 12 | 5.70 | KGATIDQPDHRG | Rv0569 | |
| 1163.530 | Hypothetical protein Rv0569 | 9517 | 4 | 5.70 | RSSDGSPPYVVRW | Rv0569 | |
| 1269.646 | Hypothetical protein Rv0569 | 9517 | 17 | 5.70 | RFGAVQSAILHARG | Rv0569 |