Table 1.
Analysis of results of tests performed for detection of HCMV with the donor-renal recipient patients
| Groups | Donor/Recipient | No of pairs positive by real time PCR | No of pairs positive for pp65 antigenemia |
|---|---|---|---|
| Group A | *D+/PRR-/PSR+ | 3/3 | 0/3 |
| Sub-Group I | |||
| Sub-Group II | D-/PRR-/PSR+ | 26/26 | 15$/26 |
| Sub-Group III | *D+/PRR+/PSR+ | 1/1 | 0/1 |
| Sub-Group IV | *D+/PRR-/PSR- | 2/2 | 0/2 |
| Sub-Group V | D-/PRR-/PSR- | 0/7 | 0/7 |
| Group B | D-/PRR-/PSR+ | 6#/18 | 2/18 |
| D+/PPR-/PSR+ | 1/18 | 0/18 | |
| Group C | PSR+ | 19^/19 | 19/19 |
| D+ | 4/19 | 0/19 | |
In Group A,
*- Donor (D), pre transplantation renal recipient (PRR) and post-transplant recipient (PSR) samples.
- Post transplant recipients were tested for HCMV infections by pp65 antigenemia and viraemia by real-time PCR.
- All the donors were pp65 antigenemia negative indicating that they were not infective.
- * Six donors belonging to sub-groups I, III and IV were positive for HCMV by real time - PCR with copy numbers ranging from 1 to 537 copies/ml were all negative for HCMV DNA.
Group B consisted of donors from whom sampling was not possible after one month
of renal transplantation.
Group C consisted of unrelated donors and recipients.
- # Two among the recipients were positive for pp65 antigenemia also.
- ^ All 19 positive for HCMV DNA were also positive for pp65 antigenemia also.
- $ All 15 patients samples positive for pp65 antigenemia were also positive for real time
PCR also.
- Eleven more were positive only by real time PCR assay.